mouse egr1  (Novus Biologicals)

Journal: Oncology Reports

Article Title: Lentinan induces apoptosis of mouse hepatocellular carcinoma cells through the EGR1/PTEN/AKT signaling axis

doi: 10.3892/or.2023.8579

Figure Lengend Snippet: Effects of LNT on the EGR1/PTEN/AKT axis in Hepa1-6 cells. (A) Protein expression levels of EGR1, PTEN, p-Akt and phosphorylation of Akt after treatment with a gradient of LNT concentrations as detected by WB. All data for protein expression were normalized using β-actin as a loading reference. (B) Immunofluorescence co-staining was used to assess the localization and expression of EGR1 and PTEN in Hepa1-6 cells treated with a gradient of LNT concentrations. Images were observed at ×630 magnification. Scale bars, 50 µm (white). (C) WB was used to detect the expression of EGR1 in the nuclear and cytoplasmic fractions following treatment with a gradient of LNT concentrations. (D) WB was used to detect the expression of EGR1, PTEN, p-Akt, and phosphorylation of Akt after EGR1 overexpression. *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. LNT, Lentinan; EGR1, early growth response 1; p-, phosphorylated; PARP1, poly-(ADP ribose) polymerase 1; Hsp60, heat shock protein 60; OE, overexpression; WB, western blotting.

Article Snippet: Subsequently, they were incubated with a mixture of different primary antibodies, including mouse EGR1 (1:100; cat. no. H00001958-M03; Novus Biologicals, LLC) and rabbit PTEN (1:100; cat. no. AF6351; Affinity Biosciences), overnight at 4°C.

Techniques: Expressing, Phospho-proteomics, Immunofluorescence, Staining, Over Expression, Western Blot

Journal: Oncology Reports

Article Title: Lentinan induces apoptosis of mouse hepatocellular carcinoma cells through the EGR1/PTEN/AKT signaling axis

doi: 10.3892/or.2023.8579

Figure Lengend Snippet: Inhibitory effect of LNT on DEN-induced primary liver cancer in mice. (A) The expression levels of EGR1, PTEN and other proteins in the liver tissues of the normal group and the model group, as analyzed by WB. (B) Expression of EGR1, PTEN and other proteins in liver tissues of the model and LNT-treated groups, as analyzed by WB. (C) Immunohistochemical analysis of EGR1, PTEN and Ki-67 in the tissue sections. Magnification, ×400. Scale bars, 200 µm (black). *P<0.05, **P<0.01, ***P<0.001 and ****P<0.0001. LNT, Lentinan; DEN, diethylnitrosamine; EGR1, early growth response 1; WB, western blotting; p-, phosphorylated; PCNA, proliferating cell nuclear antigen.

Article Snippet: Subsequently, they were incubated with a mixture of different primary antibodies, including mouse EGR1 (1:100; cat. no. H00001958-M03; Novus Biologicals, LLC) and rabbit PTEN (1:100; cat. no. AF6351; Affinity Biosciences), overnight at 4°C.

Techniques: Expressing, Immunohistochemical staining, Western Blot

Journal: Oncology Reports

Article Title: Lentinan induces apoptosis of mouse hepatocellular carcinoma cells through the EGR1/PTEN/AKT signaling axis

doi: 10.3892/or.2023.8579

Figure Lengend Snippet: Possible mechanism of LNT against liver cancer through the EGR1/PTEN/AKT axis. Blunt arrows indicate that the signaling pathways are inhibited, whilst pointed arrows indicate that the signaling pathways are activated. LNT, Lentinan; EGR1, early growth response 1.

Article Snippet: Subsequently, they were incubated with a mixture of different primary antibodies, including mouse EGR1 (1:100; cat. no. H00001958-M03; Novus Biologicals, LLC) and rabbit PTEN (1:100; cat. no. AF6351; Affinity Biosciences), overnight at 4°C.

Techniques: Protein-Protein interactions

Journal: bioRxiv

Article Title: Dysregulation of neuronal activity-dependent immediate early genes in a mouse model of Angelman syndrome

doi: 10.1101/2025.04.15.648865

Figure Lengend Snippet: Both WT and AS mice were sacrificed, and their age of P9 (before eye opening) and P25 (after eye-opening), visual cortex were carefully removed and processed for immunoblot analysis using antibodies against Arc, Egr1, Egr3, Ube3a, and β-actin. Band intensities of the blots were quantified, normalized against β-actin, and expressed as fold change. A) Immunoblot data. B) Decreased levels of Arc, Egr1, and Egr3 in the AS mice visual cortex at P25 compared to WT mice. Values represented are mean ± SEM with three mice samples in each group. * P < 0.001 compared to P25 WT group (One-way ANOVA followed by post hoc test). Each lane in the immunoblot represents a sample from different mice. C, D) Protein and mRNA levels of Ube3a were significantly increased in the visual cortex of WT mice after eye opening at P25 when compared to before eye opening at P9. C) Quantitation of immunoblot data of Ube3a shown in A. D) Quantitative RT-PCR analysis of Ube3a. In C and D, values are mean ± SEM with 4-6 animals in each group. * P < 0.01 compared to P9 age group ( t -test).

Article Snippet: Mouse monoclonal antibodies against Egr1 (sc-515830), Ube3a (sc-16689), Arc (sc-17839), and Egr3 (sc-390936) were purchased from Santa Cruz Biotechnology.

Techniques: Western Blot, Quantitation Assay, Quantitative RT-PCR

Journal: bioRxiv

Article Title: Dysregulation of neuronal activity-dependent immediate early genes in a mouse model of Angelman syndrome

doi: 10.1101/2025.04.15.648865

Figure Lengend Snippet: Brain sections with visual cortical area obtained from WT and AS mice (P25) were placed on the same slide and subjected to immunofluorescence staining with Arc, Egr1, Egr3, and Ube3a antibody followed by fluorescence-conjugated secondary antibody. Representative images of Ube3a, Arc, Egr1 and Egr3 are shown. Three mice from each group were analysed for immunofluorescence staining. Scale bar: 50 µm.

Article Snippet: Mouse monoclonal antibodies against Egr1 (sc-515830), Ube3a (sc-16689), Arc (sc-17839), and Egr3 (sc-390936) were purchased from Santa Cruz Biotechnology.

Techniques: Immunofluorescence, Staining, Fluorescence

Journal: bioRxiv

Article Title: Dysregulation of neuronal activity-dependent immediate early genes in a mouse model of Angelman syndrome

doi: 10.1101/2025.04.15.648865

Figure Lengend Snippet: Both WT and AS mice were nurtured either in the presence of normal rearing (NR, 12 h light/12 h dark cycle) or dark rearing (DR, constant darkness up to P25), sacrificed at P25, visual cortex were collected and subjected to immunoblot analysis using antibodies against Ube3a, Arc, Egr1, Egr3, and β-actin. Band intensities were quantified, normalized with β-actin, and plotted as fold change. A) Immunoblot data. B) Quantitation data of Arc, Egr1 and Egr3. C) Quantitation data of Ube3a. Values are mean ± SEM with samples from 3 animals in each group. In B, * P < 0.001 compared to the normal rearing NR-WT group (One-way ANOVA followed by post hoc test). Please note that the levels of Egr1, Egr3, and Arc are not significantly different between NR-AS and DR-AS groups. In C, * P <0.001 compared to the NR-WT group ( t -test). Each lane in the immunoblot denotes a sample from different mice.

Article Snippet: Mouse monoclonal antibodies against Egr1 (sc-515830), Ube3a (sc-16689), Arc (sc-17839), and Egr3 (sc-390936) were purchased from Santa Cruz Biotechnology.

Techniques: Western Blot, Quantitation Assay

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: (A) FBF-1 and FBF-2 are redundant for three germline roles. GSC, germline stem cell. (B) Adult gonad organization. Progenitor Zone (PZ, yellow) includes a pool of GSCs adjacent to the distal end (asterisk) and GSC daughters making the sperm to oocyte cell fate switch (s/o switch) more proximally; mature sperm (green) are in spermatheca and oocytes develop in proximal arm (magenta). The distal end is capped by the stem cell niche. (C-F) Representative z-projection images of extruded adult gonads; stained for sperm (α-SP56, green), oocytes (α-RME-2, magenta) and DNA (DAPI, cyan). Dotted line marks gonad boundary; asterisk marks distal end; yellow line marks PZ extent. 20μm scale bar in (C) applies to (C-F) (G) Gonad stained by smFISH for lst-1 RNA, a GSC marker (yellow). 20μm scale bar in (G) applies to (G-H). (H) Gonad stained with α-phospho-histone H3 (PH3) to visualize M-phase chromosomes (yellow) and DAPI (cyan). (I) Y479A-only germline phenotype. GSCs, germline stem cells maintained; s/o switch, sperm to oocyte switch occurs, n, number gonads scored. See also Figure S6.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal αFLAG M2 Sigma-Aldrich Cat# F1804, RRID:AB_262044 mouse monoclonal αGFP 3E6 Thermo Fisher Scientific Cat# A-11120, RRID:AB_221568 mouse monoclonal αV5 Bio-Rad Cat# MCA1360, RRID:AB_322378 rabbit polyclonal αGLD-1 Betsy Goodwin (Jan et al., 1999) 87 N.A. mouse monoclonal αSP56 Sam Ward (Ward et al., 1986) 88 N.A. rabbit polyclonal αRME-2 Barth Grant (Grant and Hirsh, 1999) 89 N.A. mouse monoclonal αPH3 Cell Signaling Technology Cat# 9706, RRID:AB_331748 mouse monoclonal αGAPDH Proteintech Cat# 60004-1-Ig, RRID:AB_2107436 donkey polyclonal αMouse-Alexa647 Molecular Probes/Invitrogen Cat# A-31571, RRID:AB_162542 donkey polyclonal αRabbit-Alexa 488 Molecular Probes/Invitrogen Cat# A-21206, RRID:AB_2535792 goat polyclonal αMouse-HRP Jackson ImmunoResearch Labs Cat# 115-035-003, RRID:AB_10015289 Bacterial and Virus Strains E. coli: OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041969 E. coli: OP50-1 (Streptomycin resistant OP50) Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041971 E. coli: DH5α Competent Cells Thermo Fisher Scientific Cat# 18265017 E. coli: BL21(DE3) Competent Cells - Novagen Millipore Sigma Cat# 69450 Chemicals, Peptides, and Recombinant Proteins Alt-R TM S.p.

Techniques: Staining, Marker

Journal: Developmental cell

Article Title: PUF partner interactions at a conserved interface shape the RNA binding landscape and cell fate in Caenorhabditis elegans

doi: 10.1016/j.devcel.2024.01.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies mouse monoclonal αFLAG M2 Sigma-Aldrich Cat# F1804, RRID:AB_262044 mouse monoclonal αGFP 3E6 Thermo Fisher Scientific Cat# A-11120, RRID:AB_221568 mouse monoclonal αV5 Bio-Rad Cat# MCA1360, RRID:AB_322378 rabbit polyclonal αGLD-1 Betsy Goodwin (Jan et al., 1999) 87 N.A. mouse monoclonal αSP56 Sam Ward (Ward et al., 1986) 88 N.A. rabbit polyclonal αRME-2 Barth Grant (Grant and Hirsh, 1999) 89 N.A. mouse monoclonal αPH3 Cell Signaling Technology Cat# 9706, RRID:AB_331748 mouse monoclonal αGAPDH Proteintech Cat# 60004-1-Ig, RRID:AB_2107436 donkey polyclonal αMouse-Alexa647 Molecular Probes/Invitrogen Cat# A-31571, RRID:AB_162542 donkey polyclonal αRabbit-Alexa 488 Molecular Probes/Invitrogen Cat# A-21206, RRID:AB_2535792 goat polyclonal αMouse-HRP Jackson ImmunoResearch Labs Cat# 115-035-003, RRID:AB_10015289 Bacterial and Virus Strains E. coli: OP50 Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041969 E. coli: OP50-1 (Streptomycin resistant OP50) Caenorhabditis Genetics Center (CGC) RRID:WB-STRAIN:WBStrain00041971 E. coli: DH5α Competent Cells Thermo Fisher Scientific Cat# 18265017 E. coli: BL21(DE3) Competent Cells - Novagen Millipore Sigma Cat# 69450 Chemicals, Peptides, and Recombinant Proteins Alt-R TM S.p.

Techniques: Virus, Recombinant, Protease Inhibitor, Software, Sequencing

Journal: iScience

Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

doi: 10.1016/j.isci.2024.110306

Figure Lengend Snippet: EGF drives a hematopoiesis transcriptional program in aged HSCs (A) Schematic representation of systemic administration of EGF or saline to young and aged C57Bl/6 mice daily × 3 days, followed by isolation of BM 34 − KSL cells for single cell RNA sequence analysis. (B) Uniform manifold approximation and projection (UMAP) plot of BM 34 − KSL cell clusters ( n = 1,693) with labels assigned to each HSC cluster based on gene ontology term pathway enrichment analysis. (C) UMAP density graphs of BM CD34 − KSL distribution for each of the 4 experimental groups. (D) Dot plots of expression level and percent expression of hematopoiesis genes in BM 34 − KSL cells from the treatment groups shown. Size of dot indicates the numbers of cells that express the gene and the color indicates the expression level. (E) Dot plots of average and percent expression of aging associated genes in the groups shown. (F) Dot plots of average and percent expression of DNA repair genes in the groups shown. (G) Violin plots showing expression of Egr1 from single cell RNA-seq analysis of HSCs from young and aged mice treated with EGF or saline. (H) Violin plots of Pten expression in HSCs from young and aged mice treated with EGF or saline. (I) Expression of Egr1 in aged BM 34 − KSL cells cultured for 48 h with EGF or PBS ( n = 10/group, t test). (J) Mean numbers of CFCs in BM 34 − KSL cells cultured × 72 h with media alone, media + EGF, Media + EGF + VO-OHpic, or media + VO-OHpic alone ( n = 4 replicates per group, 250 cells/dish, ∗ p < 0.05 for total CFCs, ˆˆˆ p < 0.001 for CFU-GMs, ### p < 0.001 for CFU-GEMMs). Data represented as means +/− SEM.

Article Snippet: Mouse Egr1 FAM , ThermoFisher , Mm00656724_m1.

Techniques: Saline, Isolation, Sequencing, Expressing, RNA Sequencing, Cell Culture

Journal: iScience

Article Title: Epidermal growth factor augments the self-renewal capacity of aged hematopoietic stem cells

doi: 10.1016/j.isci.2024.110306

Figure Lengend Snippet:

Article Snippet: Mouse Egr1 FAM , ThermoFisher , Mm00656724_m1.

Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Sequencing, Software

Journal: Frontiers in Cell and Developmental Biology

Article Title: Regulation of otic neurosensory specification by Notch and Wnt signalling: insights from RNA-seq screenings in the embryonic chicken inner ear

doi: 10.3389/fcell.2023.1245330

Figure Lengend Snippet: A list of constructs used in the study.

Article Snippet: EGR1 , pEgr1 , pcDNA3.1 , Full length mouse Egr1 , Addgene (ID#11729) Eileen Adamson .

Techniques: Construct, Control, Membrane